I was gonna try doing a some cloning when I get some nice ones growing but want to try one plate with the spores also do you micropore the sides like go around the plate after injecting
2-3 weeks.
I donāt do the sides. It would probably be better to use parafilm if you wanted to do that, though micropore would get the trick done.
Nice progress!
I hear ya on yesterdayās events. Without getting too political because I know thatās frowned upon here, I will say that incitement of criminal acts is a federal offense and Iām interested what happens next.
Inoculated 2 grain bags with liquid culture maybe 5 days ago. I have nice fluffy white mycellium showing this morning. Yay! Expect it will take a few weeks for them to colonize.
Haha right! All the waiting in these hobbies is killing me inside. Speaking of. Got some nice growth in the bulk spawn. No weirdness yet. I started with very little cake, only one half pint, no mycelium was showing.
Wish I took some baseline pictures, but was in a rush because of potential contam. Will check again in two days.
I was just reading about applying the casing layer and Iām reading that Iām supposed to let it fully colonize, then add the casing layer, then put it back in the dark and let that colonize, then fruitā¦so if the casing layer was going to be the same stuff as the spawnā¦whatās the pointā¦isnāt it just a thicker version of the same thing?
Interesting. I havenāt heard of this. Iāve always cased when I initiate tub colonization. When it colonizes, I fruit it.
I picked up a quart of hydrogen peroxide today for a dollar at a Meijer (big box grocery) and it wasnāt a sale price. Itās probably widely available now if anyone needs some.
Yeah thatās what I did then started reading and got confused. Whatever Iām gonna roll with what I have. Then fruit it when itās white and see what happens. I really donāt expect this to work anyway but if it does sweet.
Nothing is happening in any of these bags or jars whatās going on itās been about two weeks on some and a week on the others being sterile as hell doing in still air box donāt know whatās going on
Damn that sucks to hear. If youāre confident in everything procedurally and environmentally thereās really only one place to lookā¦the spores. Iām seeing action in about five days at ~70f
These new spores looked good Iām gonna try the site you recommend next itās pissing me off though Iām using 10 percent bleach to clean bathroom and still air box alcohol showering heating the needle tip everything then putting them in back container so no light coming through heat mat in their set to 72
Are you letting your needle tip cool down enough? Seems like a simple step but could kill them on the way through.
My other thought is brf 1/2 pints are pretty foolproof. Iām getting away with murder compared to how careful you guys are being. At the loss of ten bucks and .5 ml of spore solution maybe give one of them a shot. Stem sterilize for 90 mins hasnāt failed me. Hour or less gets occasional contam.
Yeah letting it cool wiping with alcohol too before inject injecting
Iāve seen your comments and can tell you know what your doing so Iād definitely be looking at the spores. You guys all agree that one place sucks, which one did you deal with this time?
These were from quality spores actually syringes looked nice too nice back spores definitely gonna try mushroom.com next
Sucks! I wouldnāt toss em yet you never know. Hope you get it worked out.
Is two ccs too much for. A qt jar. Maybe thatās it idk if itās too much or should I go lighter