You spawn it just like spores. You can put them in agar or liquid culture.
You need to take sterile practices up a level, but consensus seems to be cloning is the best way to go. MSS is a crap shoot with so much variability so if you want a more even flush - cloning.
Most seem to like liquid culture as @MidwestGuy mentioned or Agar. If you’re super sterile I think you can even go straight to grain. I’ve seen some cut a small chunk from the inside of a mushroom and go right into one of those microwave grain bags. Madness…
This will definitely be on the list of things to try. I’m still waiting on mycelium to spread throughout. Baby steps.
I’ve never done cloning, but I’ve done plenty of liquid cultures with spores.
You can make a spore syringe from a spore print with just RO or distilled water basically as well, correct?
Liquid culture involves like a honey mix or similar where the myc starts growing right in it, yes?
Tip: I like to pour my liquid cultures over my bulk spawns when I set them up. It really seems to accelerate the colonization of bulk spawns.
I’ve always used spores in a sterile environment for LCs, and yes, with distilled or RO. It’s helpful to PC your LC jars and let them cool before inoculating them. I use Karo syrup instead of honey. A couple ml of Karo is plenty in a half-pint jars. They grow out pretty quickly.
Another tip I’ve seen a lot, but don’t have experience with…
Don’t “throw away” a contaminated cake or grain bag etc… Find a spot and bury it shallow outside. Often times nature will nurture and you’ll have some little friends start growing.
I throw my completed spawns in my backyard in the garden. I’ve never had a shroom come up, but others’ may have success with it depending on the environment.
I mean, why not, right? A slim chance is still better than none.
Sure can it needs to be done in the most sterile of environment and should only be taken from a sample that you deem worthy of a genetic clone from. Let’s say the best looking and most beautiful, healthy looking shroom from your tub.
And it definitely needs to go to agar first to see if your sample is possibly contaminated. A lot of work goes into doing gene sampling, cloning, agar etc. You can’t be too sterile there’s no such thing.
My best b+ liquid culture came from me injecting 10cc’s of sterile water into a quart jar I was about to put to substrate pulling it back and putting that 10cc’s to a half filled pint jar that had been put through a pressure cooker at 15 psi for 30 minutes I also used honey . I’ve made 3 other pint jars from the first one I made and haven’t had a contam yet. No such thing as too sterile ever!!! Trying to work out a better set of agar samples with it now
Forgot to mention Ketchup cups! Hundreds and hundreds of ketchup cups ( Jell-O shot cups ) Just doing agar let alone making Jell-O shots with steeped, powdered shrooms.
Did an experiment I inoculated blue meanies cubensis an b+ into 3 uncle Benny’s rice not the brown rice one 2 I haven’t checked yet but 1 I opened it I had left them alone for more than a month. Right when I opened it up I saw black mold but don’t know in picture whether its mycelium or what they look like small bumps on rice? I am now trying uncle Benny’s brown rice to colonize then putting it in a fruiting chamber if I am successful at it
I checked my jars a couple days ago and not much has changed with the three. Should I rehydrate and start the fruiting process?
So I went ahead and took two jars and began rehydrating the cakes. Not sure if was the right time but I gave it a shot. Tomorrow morning I’ll roll them in vermiculite and put them in my fruiting chamber. Hopefully in a few days I’ll have some pins.
Where can I order quality spores?